Yeast Two-Hybrid Screening

Yeast Two Hybrid Screening Service

Unraveling Protein Interactions

Yeast two-hybrid (Y2H) is a classic molecular biology technique for studying protein-protein interactions within cells. It was first proposed and initially established by Fields and others in 1989 during their research on the transcriptional regulation of eukaryotic genes. With the development of molecular biology, several techniques have been developed based on the high throughput yeast two hybrid system, including yeast one-hybrid, membrane-based yeast two-hybrid, and yeast three-hybrid, which are used to study interactions between proteins, as well as between proteins and DNA/RNA/ligands. These techniques play a significant role in functional genomics and interactomics research.

Technological Superiority

High-throughput screening swiftly identifies proteins interacting with a target.

Reliable results from yeast cell expression systems reflect in vivo conditions.

High sensitivity captures even weak or transient protein interactions.

Why Choose Omics Empower?

01

Experienced

With extensive experience in screening various types of bait genes, we design suitable screening plans.

02

Genuine and Reliable

We carefully explore the appropriate screening conditions for each bait from our customers; through one-on-one spreading and spotting verification, the probability of false positives is low; we provide the delivery of positive clone materials.

03

Large-scale Library Screening

We do not skimp on the number of plates laid out, ensuring a good growth environment for positive clones.

04

Multiple Sequencing Options

Choose between first-generation sequencing or second-generation sequencing based on the number of positive clones.

05

Professional Bioinformatics Team

For less common species, we can provide functional annotations for positive clones.

06

Aesthetic Experimental Images

We can provide results images at the level of CNS articles.

Bait: We provide the CDS sequence of the bait protein or a well-constructed plasmid vector.

Library: For the nuclear system yeast two-hybrid, a library plasmid or yeast working solution is required; for the membrane system yeast two-hybrid, a library plasmid is provided.

If you want to know more about yeast two hybrid technology, please visit our website.

We are a global gene sequencing company specializing in multi-omics solutions for life science research. With a strong foundation, we have supported 2,800+ research institutions and tens of thousands of clients, contributing to 5,000+ publications with a cumulative Impact Factor nearing 40,000.